Module 6 Lesson 2 - 5 (Lab)
Lesson 2 — Mendel's Laws and Monohybrid Crosses
Lab: Drosophila Genetics (One Trait)
Introduction

Many traits have two clear phenotypes, such as tall or short pea plants. Sometimes these traits are controlled by a single gene with two different alleles. If there is complete dominance, one allele is dominant and the other recessive. When an organism has two copies of the dominant allele, or one dominant allele and one recessive allele, the dominant trait is expressed, such as green pea colour. Only when the organism has two copies of the recessive allele does the recessive phenotype come through, such as yellow pea colour.
In this simulation, you will explore Drosophila wing length colour as a single gene trait. You will cross pure breeding parents to produce an F1 generation, and then cross flies from the F1 generation to produce the F2 generation. By the end of this activity you should have a good understanding of how dominant and recessive alleles perform and how to determine the expected genotypic and phenotypic ratios for a monohybrid cross involving complete dominance.
Scientific Background
The patterns of inheritance shown by the Drosophila wing size lab were discovered originally by Gregor Mendel. After attending the University of Vienna, Mendel became interested in pea plants to study the mode of inheritance. Pea plants have many phenotypic traits that are easy to observe and are determined by a single gene. After years of experiments, Mendel discovered two remarkable patterns. The heterozygous offspring (F1) of two pure breeding homozygous parents all resemble one parent. If two of the F1 heterozygous offspring are bred, 75% of their offspring (F2) show the dominant trait while 25% shows the recessive trait. These patterns helped Mendel formulate his theory of inheritance and begin the modern science of genetics.Problem
Cross P1 purebreeding flies and F1 flies and observe the resulting offspring to determine how dominance and recessive alleles produce a recognizable inheritance pattern. How can understanding this pattern allow you to predict the outcome of various new crosses?
Materials
For this simulation, you require access to the Internet and a word processing program or a blank piece of paper to record your results. You also record your results in Assignment 6D.
Procedure
- Open the link to Drosophila Wing Shape Lab
- Click on the Procedures tab on the right side of your screen.
- Follow the procedures for the P1 Generation Cross and the F1 Generation Cross.
- Record your predictions and results (see question 3 in Assigment 6D).
Results
Record your results in your observation tables in Assignment 6D (question 3)Analysis
Complete questions 4 and 5 in Assignment 6D.